Toxicology Research Today is a free monthly online journal that collates and summarizes the latest research about Toxicology, including details on forensic toxicology, carcinogenicity, assays.

Two-dimensional gels for toxicological drug discovery applications.

Witzmann FA, Richardson MR

1Professor of Cellular and Integrative Physiology, Indiana University School of Medicine, Department of Cellular & Integrative Physiology, Biotechnology & Research Training Center, 1345 West 16th Street Room 308, Indianapolis, IN 46202, USA. fwitzman@iupui.edu , 2Pre-doctoral Fellow, Indiana University School of Medicine, Department of Cellular & Integrative Physiology, Biotechnology & Research Training Center, 1345 West 16th Street Room 308, Indianapolis, IN 46202, USA.

Two-dimensional gel electrophoresis (2DGE) continues to be a useful approach to study protein expression. Although liquid chromatographic and mass spectrometric approaches that overcome some of the limitations and labour intensity of 2DGE are increasingly popular, this electrophoretic approach still has exceptional relevance in toxicology. Despite the technical challenges, pharmacologists/toxicologists continue to use gel-based proteomics to assess the biological and health effects of chemical treatment and exposure. This brief review addresses the use of 2DGE-based proteomics in drug development and toxicology, emphasising its unique strengths and weaknesses, and considers recent developments in this strategy that have evolved to directly confront the issues of dynamic range and reproducibility that have previously limited the overall use of 2D electrophoresis.

Published 25 July 2006 in Expert Opin Drug Metab Toxicol, 2(1): 103-111.
Full-text of this article is available online (may require subscription).

© 2005-2008 Toxicology Research Today. All Rights Reserved.